We can even customize your database to track facilityspecific organisms. Standard operating procedure manual with pm records and logs. Microbial identification using the biomerieux vitek 2 system david h. Performance of vitek2 compact and overnight microscan. Biomerieux, the biomerieux logo, vitek, advanced expert system, vilink, myla, and densichek. Etest is a quantitative technique for determining the antimicrobial susceptibility of gramnegative and grampositive aerobic bacteria such as enterobacteriaceae, pseudomonas, staphylococcus and enterococcus species and fastidious bacteria, such as anaerobes, n. For isolates grown on agar, place a drop of sterile distilled water or pbs onto the center of a clean cover slip. Download our vitek 2 compact procedure manual ebooks for free and learn more about vitek 2 compact procedure manual. All isolates were tested with gram negative susceptibility. Coffee maker vitek vt1508 r manual instruction 52 pages summary of contents for vitek vt1506. Anp and bnp, by means of their natriuretic and diuretic properties, and as antagonists of the reninangiotensinaldosterone system, influence the electrolyte and fluid balance in an organism.
The fundamentals of vitek 2 compact operations are presented through lessons, tutorials and exercises to enable you to take the most out of the instrument. Todays global healthcare challenges like multidrug resistant organisms mdro mean that microbiology labs need to be flexible and responsive to provide the right information at the right time. All users of the vitek 2 compact v2c must have hands on training in the use of the instrument. This new card allows the identification of 159 taxa. Manufacturer specifications vitek 2 compact, biomerieux please note. No reagent addition needed with closed, readytouse disposable system. The vitek densichek instrument is intended for professional use by laboratory health professionals ex. The vitek 2 advanced expert system software is like having an expert advisor standing by your side. The efficiency of the vitek 2 compact instrument and vitek 2 pc software offers you the capacity to help improve therapeutic success and patient outcomes 1 through reliable microbial.
Verification and validation in clinical microbiology. Safety is optimized with minimal reagent preparation and reduced handling in a closed, disposable system. Vitek susceptibility test results are expressed as minimum inhibitory concentration mic values and interpreted as susceptible, intermediate or resistant by reference to a clsi. Microbiology laboratory technologists in clinical or industry settings. Vitek 2 compact system direct testing procedure idgn and astn 020 cards were used, respectively, for identification and susceptibility testing directly from positive bactalert fa and fn blood cultures. Vitek 2 compact identifies the vast majority of microorganisms that contaminate production areas and finished products in a minimum amount of time.
Vitek 2 compact instrument user manual chapter, guidance to select a vitek 2 identification card. The identification of all cns isolated in the procedure was firstly made by vitek 2 compact according to the manufacturers manual. The vitek 2 compact nextgeneration platform provides greater automation while increasing usersafety and eliminating repetitive manual operations. It ranged from 95% for grampositive cocci to 98,1% for gramnegative rods. This instrument uses the same idast cards, data management software and advanced expert system aes as the larger vitek 2 instrument. The aim of this study was to compare the application of phenotypic and molecular tools in bacterial identification on the example of gramnegative. Application of routine diagnostic procedure, vitek 2. Administrator or supervisor level login access is required. The innovative vitek 2 technology includes an expanded identification database, the most automated platform available, rapid results, improved confidence, with minimal training time. Vitek 2 xl vitek 2 compact vitek 2 0118 93192002gbe document andor pictures not legally binding. Designed to provide idast results in as little as 5 to 8 hours3, vitek 2 compact works with economical, readytouse vitek 2 idast cards.
Vitek 2 system in clinical laboratories as an in vitro test to determine the susceptibility of gram positive organisms to vancomycin when used as instructed in the system product information manual. From these data, the phoenix ast results demonstrated a high degree of agreement with all systems evaluated, although fewer vmes were observed with the. We own biomerieux vitek 2 user manual pdf, epub, doc, djvu, txt forms. Jan 25, 2016 in diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. To inoculate the vitek 2 cards directly from a positive blood culture bottle, a 5ml aliquot was centrifuged at 60 g for 10 min. Access free vitek 2 compact manual vitek 2 compact manual. The vitek 2 system nextgeneration platform provides greater automation while increasing safety and eliminating repetitive manual operations. Vitek 2 instrument user manual i 51073110en1 general warnings important.
The vitek 2 system correctly identified to the species level only one choice or after simple supplementary tests 99% of s. The user is advised to read and understand all instructions in this manual to be able to derive the best performance from the vitek 2 instrument and the smart carrier station. A high rate of id agreement 96,5% between vitek 2 compact and the conventional methods was observed. If in exceptional cases the barcode cannot be read, enter the 15digit sequence of numbers. Biomerieux vitek 2 compact automated microbial identification and. Vitek 2 yeast caspofungin cards and the acceptable range. Hazelwood, mo, usa objective this chapter describes the vitek 2 automated microbiology system and its application in the identification of microorganisms. Results can be obtained as quickly as 3 to 7 hours thanks to the combination of analysis software and an original. Vitek 2 identification id cards let you easily and safely test and identify a wide range of clinically relevant organisms. Vitek 2 compact procedure manual, vitek procedure manual, vitek 2 procedure manual. Identification and antibiotic susceptibility testing. Vitek 2 compact bacterial identification and monitoring. The vitek is an automated system for identification and antibiotic susceptibility testing.
The compact solution for fast, accurate idast testing with expert confidence. The product information manual, software user manual, industry. Though there are reports of coagulasenegative staphylococci being wrongly identified as kocuria by the vitek 2 system 21, other more recent studies indicate a good reliability of the vitek 2. Vitek2 compact system direct testing procedure idgn and astn 020 cards were used, respectively, for identification and susceptibility testing directly from positive bactalert fa and fn blood cultures. Refer to the vitek 2 systems software user manual or the vitek 2 systems web software user manual for information about accessing, creating and managing user accounts. The use of vitek 2 compact for microbial identification has been reported and endorsed by several authors quesada et al. Use of the vitek 2 system for rapid identification of. Vitek 2 technology represents a smarter way to automate idast testing. The vitek densichek can be used to prepare suspensions for testing with vitek 2 automated idast system.
To inoculate the vitek2 cards directly from a positive blood culture bottle, a 5ml aliquot was centrifuged at 60 g for 10 min. Evaluation of the vitek 2 system for identification and. Gp test kit vtk 2 card was used for identification of all the isolates already identified were stored in 20% skim milk growth medium and frozen at. Application proper use of the instrument is the responsibility of trained. Test time to result correct id gn gramnegative bacilli 2 10 hours 96. The average times to results for phoenix, vitek 2, and the manual methods were 12. Csf, body fluids, drug trough or peak, other unrecoverable specimens due to patient recollect inconvenience. The vitek 2 is an automated microbiology system utilizing growthbased technology. The vitek 2 antimicrobial susceptibility test ast is intended to be used with the vitek 2 and vitek 2 compact systems for the automated.
Us federal law restricts this device to sale by or on the order of a licensed practitioner. View and download vitek vt1506 manual instruction online. Standard operating procedure page 2 of 4 atrial natriuretic peptide anp, brain natriuretic peptide bnp, and ctype natriuretic peptide cnp. Pdf vitek 2 automated identification system and kocuria. Use, maintenance and quality control procedures scope the purpose of this sop is to describe the procedures for the preparation and identification of test microorganisms test microbes and quality control organisms using the vitek 2 compact instrument. Personnel are required to be knowledgeable of the procedures in this sop. There are over one million free books here, all available in pdf, epub, daisy, djvu and ascii text. The product information manual, software user manual, industry customer training course book and instrument user manual are maintained near the instrument. The course the routine use of vitek 2 compact has been designed for those who need to operate the instrument in a routine mode without having to perform expert tasks. Microbial identification using vitek 2 pdf pdadhi parenteral. Gp test kit vtk 2 card was used for identification of all the isolates already identified were stored in 20% skim milk growth medium and frozen at 20. Principles the vitek 2 is an automated microbiology system utilizing growthbased technology. Mar 16, 2018 this feature is not available right now.
Quality control was performed using the vitek 2 yeast caspofungin card and testing was done by both the manual dilution and the automated dilution method for vitek 2 system and by the manual dilution method only for vitek 2. These books contain exercises and tutorials to improve your practical skills, at all levels. Standard operating procedure page 2 of 4 specimens. The collector or manager must personally label the sample and provide their name and department. The configuration that you have purchased is adapted to the legislation. Designed for vitek 2 automated instruments, vitek 2 id cards help ensure you can rapidly provide accurate, relevant results. The vitek 2 compact bacterial identification system, which is available in three sizes, brings vitek 2 technology to laboratories of every size. Manufacturer specifications vitek 2 compact, biomerieux. Vitek manual table of contents mount sinai hospital. Vitek manual university health networkmount sinai hospital, department of microbiology note. After primary organism isolation, there is minimal. Automated methods for antibiotic susceptibility testing. Incubate at room temperature until growth is obtained, usually 24 hours. The innovative vitek 2 microbial identification system includes an expanded identification database, the most automated platform available, rapid results, improved confidence, with minimal training time.
Application of routine diagnostic procedure, vitek 2 compact. Performance of vitek2 compact and overnight microscan panels. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of capacity and automation. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Personnel are required to be knowledgeable of the procedures in this. A total of 426 isolates 331 fermenting and 95 nonfermenting gramnegative bacilli belonging to 70 taxa covered by the database were evaluated. In diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. Gain rapid, accurate susceptibility test results and resistance detection in clinically relevant bacteria and yeasts with selfcontained, disposable test cards for use with vitek 2 instruments. You can search for ebooks specifically by checking the show only ebooks option under the main search box. Startup procedure 1 make sure the vitek 2 instrument has been connected to an appropriate power supply using the power cord supplied with the instrument. To find more books about vitek 2 compact procedure manual, you can use related keywords. Rapid resistance detection to aid in accurate diagnosis and prompt, effective treatment preapplied barcodes for maximum traceability closed system. Welcome to the biomerieux vitek 2 current information specific to the vitek 2 menu of options to help guide you. Click the vitek 2 system legacy icon on the desktop.
Us environmental protection agency office of pesticide programs. Standard operating procedure gundersen health system. Comparison of bd phoenix to vitek 2, microscan microstrep. From these data, the phoenix ast results demonstrated a high degree of agreement with all systems evaluated, although fewer vmes were observed with the phoenix than with the vitek 2. At biomerieux, biomerieux vitek 2 compact user manual vitek 2 compact manual books and manuals. The study has shown a high correlation of results n 1118 obtained by aid of vitek 2 compact drug sensitivity tests and the results obtained by the aid of. Modifications by biomerieux can be made without prior notice biomerieux, the blue logo, advanced expert system, bioart, densichek, myla, vilink and vitek are used, pending andor registered trademarks belonging to biomerieux, or one of its subsid iaries, or one of its. A broad choice of methods is available, based on both phenotypic and molecular properties of microbes. Vitek 2 compact, automated idast instrument biomerieux. An rl6 event andor safety zone event form will be completed.
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