Leica sp8 manual pdf

Instructions for using leica dcm3d confocal microscope. Turn on the scanner power 1 on the front of the compact supply unit csu 3. If you do not require high speed scanning leave resonant set to off. This user manual specifies names of products or services that are trademarks or registered. No 159330033 vers 01082001 click on an item means to move the pointer onto the specified item and press and release the mouse button once. Sp8 lightning confocal microscope products leica microsystems. Leica tcs sp8 technical documentation life science research division. Check to make sure antivibration table is on power switch in the back of unit 3. The leica tcs sp8 is designed to meet all of your future requirements with effectiveness and efficiency. Adjust the laser intensity of the appropriate laser lines by moving the slider up or by directly.

The leica sp8 confocal microscope is for live cell imaging with highspeed, multispectral, supersensitivity, superresolution, and fluorescence lifetime capability. User manuals, guides and specifications for your leica dmi6000b microscope. Pdf leica rm2255 ifu 2v3j en pdf leica rm2255 ifu 2v3j es pdf leica rm2255 ifu 2v3j fr pdf leica rm2255 ifu 2v3j it pdf. Leica sp8 white light confocal, gsted, flim cell sciences. The sp8 lightning allows you to perform proper sampling over a specimen area at least six times larger than with any other confocal system. This user manual provides you with important information for using the system, the necessary ambient conditions and the usable lasers. Database contains 3 leica dmi6000b manuals available for free online viewing or downloading in pdf. Page 1 user guide to the ibif leica tcs sp8 mp confocal microscope this version. It is built around the leica prismbased spectral detector system on a motorized inverted microscope stand.

User manual leica tcs sp8 leica tcs sp8 mp leica tcs sp8 x. The microscope is suitable for both live cell and fixed specimens on slides, and it will. You must have additional training to use the advanced features on this system. The leica sp8x smd confocal microscope is located in room 00075, lab 1. Leica m typ 240 camera user manual, instruction manual, user guide pdf free download leica m typ 240 pdf user manual, user guide, instructions, leica m typ 240 owners manual. Tailor your microscope to your current needs and upgrade with additional functionalities at any time.

Double click on the las x software found on the desktop. Leica dm2500 upright fluorescent microscope manuals. This user manual specifies names of products or services that. Quick operation manual for leica sp8 confocalmultiphoton. Leica tcs sp8 x the only freely tunable confocal system the white light laser source of the leica tcs sp8 x perfectly matches the spectral properties of any fluorophore excitable in the visible spectral range. Live cell imaging or light sheet mode that exceeds a 4hour duration must be conducted at. View and download leica tcs sp8 mp user manual online. Leica sp8 the leica microsystems sp8 confocal with digital light sheet has the following features and capabilities. This user manual specifies names of products or services that are trademarks or. Usermanual for leica sp8 confocal contact information. Training guide for leica sp8 confocalmultiphoton microscope.

Operational training manual sp8 quick start instructions. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed. The leica sp8 x smd confocal microscope is located in room 00075, lab 1. The gain setting as discussed elsewhere in this manual controls the sensitivity. Leica tcs sp8 technical documentation en 11 2016, pdf, 499. Leica biosystems division of leica microsystems inc. Turn on the ctr control box, el6000fluorescent light source for the. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk. The microscope is suitable for both live cell and fixed specimens on slides, and it will run alongside the old zeiss lms 510 metahead confocal. Contents startup preparing for imaging part 1 on the scope. Frap with resonant scanner appletter en, pdf, 2 mb. View and download leica sp8 training manual online. Never again compromise on speed, resolution and sensitivity.

Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been trained. Quick operation manual for leica sp8 confocalmultiphoton microscope. Confocal imaging using a super continuum, white light wll pulsed laser source that allows users to specify any excitation wavelength between 470nm and 670nm. Leica sp8 resonant confocal nrimcdb microscopy facility. User manual leica tcs sp8 smd for fcs, flim and flcs. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy. A guide through the live data mode live data mode with leica tcs sp8 5 3 definition of an experiment some experiments can be more complex and require differ ent hardware settings at a certain time within the course of the experiment. If you turn it on you will not be able to use slower speeds. Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user, with the. Together with an aobs acoustical optical beam splitter up to 8 discrete laser lines can be selected simultaneously with a 1 nm precision. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Leica m9 m9p is the mmount fullformat digital rangefinder camera equipped with a 18 mp ccd sensor developed specifically for this camera, enables the capture of. The leica microsystems sp8 confocal with digital light sheet has the following features and capabilities.

Turn on power to microscope power unit is located at your feet, next to the computer. Leica sp8 confocal, sted, flim system microscopy and. Adjust the laser intensity of the appropriate laser. Leica ssystem a class of its own leica s3 64 megapixel. Continuously tunable excitation and detection ensure best. School of life sciences these are procedures provided by leica microsystems that describe how to operate the instrument in its most simplest format. It achieves this level of performance using highspeed resonant scanning with imaging formats of up to 2496 x 2496 pixels. Leica dm2500 upright fluorescent microscope manuals cell. Your investment in a leica tcs sp8 will pay off now and in the future. For such experiments, live data mode is the tool of choice. Enable the lasers by turning the key from the off position. Leica microsystems heidelberg gmbh lcs software functions user manual leica lcs english page 8 of 164 art. For information about the licenses offered, refer to this page.

Continuously tunable excitation and detection ensure best image quality and sample protection. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. If you do not require high speed scanning leave resonant set to. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. Quick start leica tcs sp8 system start up image acquisition zseries acquisition system shut down software start up laser start up turn on the microscope control box. Leica sp8 pointscanning confocal system, on a leica dmi6000b inverted microscope, confocal laser scanning microscope leica tcs sp8 en, pdf, 535. Live cell imaging with adaptive focus control afc to automatically maintain focus in timelapse experiments.

The portable document format pdf redundantly pdf format is a file format developed by adobe in the 1990s to present documents including text formatting and images in a manner independent of application software hardware and operating systems. Navigator software module creates a fast overview of samples. Solid state lasers and the el6000 widefield light source require only minimal warm up, but adhere to 15min15min rule. Microscopes microscope stand options upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs leica dmi8 cel compact leica dmi8 cel advanced vibration isolation isolation options speci. Turning on the system 1turn on the fluorescent light source number 1 2 turn on the ndd power supply. Pdf leica rm2255 ifu 2v3j en pdf leica rm2255 ifu 2v3j es. Leica sp8 tcs users manual v2 university of oklahoma. Leica sp8 dive multiphoton microscope manualquick guide.

They prefer to invest their idle time to talk or hang out. Turn on the fluorescent lamp top left of the computer monitor. User guide created by daniel desantis and linda mayernik, october, 2015. In addition, this operating manual provides a rough overview of the. User guide to the ibif leica tcs sp8 mp confocal microscope. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. Chinese, english, french, german, indonesian, italian, japanese, korean, polish, russian, taiwanese. The leica sp8 in the keck lab has additional hyd detectors, resonant scanner, live cell. Las x live data mode recording of manual and automated workflows, trigger functions, complex timelapse series. Tltransmitted light 2 toggles the shutter between open and closed for brightfield illumination.

Then turn on the power supply for the scanner 1 and the lasers 2 by. Leica microsystems cms gmbh am friedensplatz 3 d68165 mannheim germany. User manual sp8 leica confocal janvier2016 turn on the. The leica sp8 5d is equipped with a series of five tuneable photoreceptors. Sp8 lightning konfokalmikroskop produkte leica microsystems.

Sp8 lightning confocal microscope leica microsystems. May 18, 2016 leica m9 m9p camera user manual, instruction manual, user guide pdf free download leica m9 m9p pdf user manual, user guide, instructions, leica m9 m9p owners manual. Upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs. Turn on the ctr control box, fluorescent light for the microscope stand. The leica sp8 in the keck lab has additional hyd detectors, resonant scanner, live cell imaging mode and light sheet. This screen highlights the aperture opening and the intensity of the transmitted light. Usermanual for leica sp8 confocal universitetet i bergen. Leica m typ 240 rangefinder digital camera sports a 24 mp fullframe cmos sensor delivers natural and brilliant color rendition and impressive reproduction of. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multi photon confocal microscopy.